Screening Cloned PCR Fragments by Restriction Endonuclease Fingerprinting to Obtain Wild-Type Sequences
نویسندگان
چکیده
منابع مشابه
Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences.
A major application of polymerase chain reaction (PCR) is the cloning of DNA fragments with known sequences, e.g., full-length cDNAs and genomic fragments. Although the introduction of the long PCR procedure (2) has facilitated this PCR-based cloning, its usefulness is limited by the high rates of faulty nucleotide incorporation that is seen in all heat-stable DNA polymerases (6). Although seve...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98254bm01